Research Output

Publications.

Discoveries and methodological advances in membrane protein chemistry, native mass spectrometry, molecular interactions, and structural biology.

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Selected Work

Featured publications

2026J Am Chem Soc

Time-Resolved Native Mass Spectrometry for Direct Measurement of Biomolecular Kinetics

James VK, Stover L, Bahramimoghaddam H, Khandelwal T, Chang JY, Downing J, Scott E, Bailey KO, Russell DH, Baker LA, Laganowsky A

DOI ↗ PubMed ↗

2025J Am Chem Soc

Molecular Basis for the Activation of Pseudomonas aeruginosa MsbA by Divalent Metals

Lyu J, Bahramimoghaddam H, Zhang T, Scott E, Yun SD, Yadav GP, Zhao M, Russell D, Laganowsky A

DOI ↗ PubMed ↗

2025J Am Chem Soc

Design and Synthesis of Hetero-Bicephalic Detergents for Native Mass Spectrometry of Membrane Proteins

Chang JY, Azizov D, Shinde VN, Yun SD, Rider RL, Zhu Y, Bahramimoghaddam H, Lynn M, Russell DH, Fang L, Laganowsky A

DOI ↗ PubMed ↗

2024Proc Natl Acad Sci U S A

Capturing RAS oligomerization on a membrane

Yun SD, Scott E, Chang JY, Bahramimoghaddam H, Lynn M, Lantz C, Russell DH, Laganowsky A

DOI ↗ PubMed ↗

2023Chemical Science

Native mass spectrometry of proteoliposomes containing integral and peripheral membrane proteins

Zhu Y, Yun SD, Zhang T, Chang JY, Stover L, Laganowsky A

DOI ↗ PubMed ↗

2014Nature

Membrane proteins bind lipids selectively to modulate their structure and function

Laganowsky A,Ф, § Reading E, Allison TM, Ulmschneider MB, Degiacomi MT, Baldwin AJ, Robinson CV

DOI ↗ PubMed ↗

Complete Record

Research articles

20266 publications

#122

Time-Resolved Native Mass Spectrometry for Direct Measurement of Biomolecular Kinetics

James VK, Stover L, Bahramimoghaddam H, Khandelwal T, Chang JY, Downing J, Scott E, Bailey KO, Russell DH, Baker LA, Laganowsky A

J Am Chem Soc. 2026 May 20;148(19):19567-19577. doi: 10.1021/jacs.5c21842. PMID: 42104933.

DOI PubMed

#121

Temperature and lipid composition differentially regulate KRAS assemblies on membranes

Kang J, Scott E, Yun SD, James VK, Chang JY, Bahramimoghaddam H, Downing J, Evans K, Russell DH, Laganowsky A

Chem Commun (Camb). 2026 Jun 2;62(42):10723-10726. doi: 10.1039/d6cc01853j. PMID: 42132878.

DOI PubMed

#120

Probing the Annular Lipid Belt of Membrane Proteins Using Native Mass Spectrometry

Kumar S, Laganowsky A

Methods Mol Biol. 2026;3001:165-180. doi: 10.1007/978-1-0716-5054-7_9. PMID: 42156678.

DOI PubMed

#119

Peptidomimetic α,β-Unsaturated Ethyl Esters Are Irreversible Inactivators of Human Cathepsin L and Are Potent Inhibitors of SARS-CoV-2 in Cellular Models of COVID-19

Kumar V, Drelich A, Chenna BC, Rademacher A, Kemp AM, Bishop AC, Saenkham-Huntsinger P, Scott E, Wand AJ, Laganowsky A, Tseng CT, Meek TD

J Med Chem. 2026 May 28;69(10):11797-11818. doi: 10.1021/acs.jmedchem.5c03172. PMID: 42014929.

DOI PubMed

#118

Rubisco kinetic acclimation at the holoenzyme level

Askey B, Ceminsky M, Scott E, Wang Y, Oh ZG, Azinas S, Laganowsky A, Gunn LH

Proc Natl Acad Sci U S A. 2026 Apr 21;123(16):e2519914123. doi: 10.1073/pnas.2519914123. Epub 2026 Apr 15. PMID: 41984832.

DOI PubMed

#117

Mechanism of substrate recognition and catalysis in a mammalian phosphatidylserine synthase 2

Wang L, Zhang Z, Yang H, Laganowsky A, Zhou M

bioRxiv [Preprint]. 2026 Feb 7:2026.02.06.704487. doi: 10.64898/2026.02.06.704487. PMID: 41676735.

DOI PubMed

202511 publications

#116

Dissecting Heterogeneous Populations of Protein-Complex Samples Using Direct Mass Technology

Rider RL, Hampton J, Xi Z, Lantz C, Yun SD, Liu W, Viner R, Laganowsky A, Russell DH

Anal Chem. 2025 Dec 16;97(49):27057-27063. doi: 10.1021/acs.analchem.5c05771.

DOI

#115

Effects of Hydration on Transthyretin Conformational Dynamics and Oligomerization

Hampton J, Lantz C, Rider RL, Yun SD, Laganowsky A, Russell DH

Biochemistry. 2025 Dec 2;64(23):4571-4580. doi: 10.1021/acs.biochem.5c00589.

DOI

#114

Roles of Hydration in Protein-Ligand Binding: Passive or Active Participant? J Phys Chem A

Evans KA, Sun HM, Powers M, Lantz C, Laganowsky A, Rye H, Russell DH

2025 Sep 30. doi: 10.1021/acs.jpca.5c04986. PMID: 41026809.

DOI PubMed

#113

Hydration (H2O and D2O) Dictates the Stabilities and Conformational Entropy of Transthyretin Tetramers

Lantz C, Rider RL, Kuo ST, Xi Z, Burningham E, Yun SD, Laganowsky A, Russell DH

J Am Chem Soc. 2025 Oct 1;147(39):35616-35626. doi: 10.1021/jacs.5c11055. PMID: 40965130.

DOI PubMed

#112

Molecular Basis for the Activation of Pseudomonas aeruginosa MsbA by Divalent Metals

Lyu J, Bahramimoghaddam H, Zhang T, Scott E, Yun SD, Yadav GP, Zhao M, Russell D, Laganowsky A

J Am Chem Soc. 2025 Sep 3;147(35):31488-31496. doi: 10.1021/jacs.4c18759. PMID: 40851428.

DOI PubMed

#111

Allostery without Conformational Change: A Native Mass Spectrometry Perspective

Sun HM, Evans KA, Powers M, Xi Z, Lantz C, Laganowsky A, Rye H, Russell DH

J Phys Chem B. 2025 Aug 28;129(34):8668-8679. doi: 10.1021/acs.jpcb.5c03261. PMID: 40827960.

DOI PubMed

#110

Design and Synthesis of Hetero-Bicephalic Detergents for Native Mass Spectrometry of Membrane Proteins

Chang JY, Azizov D, Shinde VN, Yun SD, Rider RL, Zhu Y, Bahramimoghaddam H, Lynn M, Russell DH, Fang L, Laganowsky A

J Am Chem Soc. 2025 Aug 6;147(31):28513-28522. doi: 10.1021/jacs.5c10841. PMID: 40709568.

DOI PubMed

#109

Temperature-dependence of membrane protein-lipid interactions in membranes

Kumar S, Downing J, Lynn M, Stover L, Lantz C, Russell DH, Laganowsky A

Chem Commun (Camb). 2025 Jul 24;61(61):11429-11432. doi: 10.1039/d5cc01576f. PMID: 40576626.

DOI PubMed

#108

Real time characterization of the MAPK pathway using native mass spectrometry

Scott E, Yun SD, Moghadamchargari Z, Bahramimoghaddam H, Chang JY, Zhang T, Zhu Y, Lyu J, Laganowsky A

Commun Biol. 2025 Apr 16;8(1):617. doi: 10.1038/s42003-025-08028-5. PMID: 40240517; PMCID: PMC12003711.

DOI PubMed

#107

Adapting a Trapped Ion Mobility Spectrometry-Q-TOF for High m/z Native Mass Spectrometry and Surface-Induced Dissociation

Lin YF, Jones BJ, Ridgeway ME, Panczyk EM, Somogyi A, Kaplan DA, Marathe I, Yun S, Kirby KA, Sarafianos SG, Laganowsky AD, Park MA, Wysocki VH

Anal Chem. 2025 Feb 16. doi: 10.1021/acs.analchem.4c03557. PMID: 39957060.

DOI PubMed

#106

Symmetry facilitated the evolution of heterospecificity and high-order stoichiometry in vertebrate hemoglobin

Cortez-Romero CR, Lyu J, Pillai AS, Laganowsky A, Thornton JW

Proc Natl Acad Sci U S A. 2025 Jan 28;122(4):e2414756122. doi: 10.1073/pnas.2414756122. Epub 2025 Jan 23. PMID: 39847336.

DOI PubMed

202417 publications

#105

Catalytic Mechanism of SARS-CoV-2 3-Chymotrypsin-Like Protease as Determined by Steady-State and Pre-Steady-State Kinetics

Zhu J, Kemp AM, Chenna BC, Kumar V, Rademacher A, Yun S, Laganowsky A, Meek TD

ACS Catal. 2024 Nov 27;14(24):18292-18309. doi: 10.1021/acscatal.4c04695. PMID: 39722883.

DOI PubMed

#104

Native Mass Spectrometry of Membrane Protein-Lipid Interactions in Different Detergent Environments

Kumar S, Stover L, Wang L, Bahramimoghaddam H, Zhou M, Russell DH, Laganowsky A

Anal Chem. 2024 Oct 22;96(42):16768-16776. doi: 10.1021/acs.analchem.4c03312. Epub 2024 Oct 12. PMID: 39394983.

DOI PubMed

#103

Symmetry facilitated the evolution of heterospecificity and high-order stoichiometry in vertebrate hemoglobin

Cortez-Romero CR, Lyu J, Pillai AS, Laganowsky A, Thornton JW

bioRxiv [Preprint]. 2024 Dec 13:2024.07.24.604985. doi: 10.1101/2024.07.24.604985. PMID: 39091803.

DOI PubMed

#102

Water Plays Key Roles in Stabilities of Wild Type and Mutant Transthyretin Complexes

Lantz C, Rider RL, Yun SD, Laganowsky A, Russell DH

J Am Soc Mass Spectrom. 2024 Aug 7;35(8):1854-1864. doi: 10.1021/jasms.4c00170. Epub 2024 Jul 26. PMID: 39057193.

DOI PubMed

#101

Biophysical Characterization of RAS-SOS Complexes by Native Mass Spectrometry

Yun S, Scott E, Laganowsky A

Methods Mol Biol. 2024;2797:177-193. doi: 10.1007/978-1-0716-3822-4_13. PMID: 38570460.

DOI PubMed

#100

Native mass spectrometry and structural studies reveal modulation of MsbA-nucleotide interactions by lipids

Zhang T, Lyu J, Yang B, Yun SD, Scott E, Zhao M, Laganowsky A

Nat Commun. 2024 Jul 15;15(1):5946. doi: 10.1038/s41467-024-50350-9. PMID: 39009687.

DOI PubMed

#99

Capturing RAS oligomerization on a membrane

Yun SD, Scott E, Chang JY, Bahramimoghaddam H, Lynn M, Lantz C, Russell DH, Laganowsky A

Proc Natl Acad Sci U S A. 2024 Aug 20;121(34):e2405986121. doi: 10.1073/pnas.2405986121. Epub 2024 Aug 15. PMID: 39145928.

DOI PubMed

#98

Biophysical Characterization of RAS-SOS Complexes by Native Mass Spectrometry

Yun S, Scott E, Laganowsky A

Methods Mol Biol. 2024;2797:177-193. doi: 10.1007/978-1-0716-3822-4_13. PMID: 38570460.

DOI PubMed

#97

TREK2 Lipid Binding Preferences Revealed by Native Mass Spectrometry

Stover L, Zhu Y, Schrecke S, Laganowsky A

J Am Soc Mass Spectrom. 2024 Jul 3;35(7):1516-1522. doi: 10.1021/jasms.4c00112. Epub 2024 Jun 6. PMID: 38843438.

DOI PubMed

#96

Native mass spectrometry of membrane protein-lipid interactions in different detergent environments

Kumar S, Stover L, Wang L, Bahramimoghaddam H, Zhou M, Russell DH, Laganowsky A

bioRxiv [Preprint]. 2024 Jun 27:2024.06.27.601044. doi: 10.1101/2024.06.27.601044. PMID: 38979331.

DOI PubMed

#95

Water Plays Key Roles in Stabilities of Wild Type and Mutant Transthyretin Complexes

Lantz C, Rider RL, Yun SD, Laganowsky A, Russell DH

J Am Soc Mass Spectrom. 2024 Aug 7;35(8):1854-1864. doi: 10.1021/jasms.4c00170. Epub 2024 Jul 26. PMID: 39057193.

DOI PubMed

#94

Grafting the ALFA tag for structural studies of aquaporin Z

Stover L, Bahramimoghaddam H, Wang L, Schrecke S, Yadav GP, Zhou M, Laganowsky A

J Struct Biol X. 2024 Feb 2;9:100097. doi: 10.1016/j.yjsbx.2024.100097. PMID: 38361954.

DOI PubMed

#93

Design of a symmetry-broken tetrahedral protein cage by a method of internal steric occlusion

Gladkov N, Scott EA, Meador K, Lee EJ, Laganowsky AD, Yeates TO, Castells-Graells R

Protein Sci. 2024 Apr;33(4):e4973. doi: 10.1002/pro.4973. PMID: 38533546.

DOI PubMed

#92

Nonlinear Frequency Modulation for Fourier Transform Ion Mobility Mass Spectrometry Improves Experimental Efficiency

Cabrera ER, Schrader RL, Walker TE, Laganowsky A, Russell DH, Clowers BH

Int J Mass Spectrom. 2024 Mar;497:117197. doi: 10.1016/j.ijms.2024.117197. Epub 2024 Jan 9. PMID: 38352886.

DOI PubMed

#91

Double and triple thermodynamic mutant cycles reveal the basis for specific MsbA-lipid interactions

Lyu J, Zhang T, Marty MT, Clemmer D, Russell DH, Laganowsky A

Elife. 2024 Jan 22;12:RP91094. doi: 10.7554/eLife.91094. PMID: 38252560.

DOI PubMed

#90

Mechanism of anion exchange and small-molecule inhibition of pendrin

Wang L, Hoang A, Gil-Iturbe E, Laganowsky A, Quick M, Zhou M

Nat Commun. 2024 Jan 6;15(1):346. doi: 10.1038/s41467-023-44612-1. PMID: 38184688.

DOI PubMed

#89

Biophysical Characterization of RAS-SOS Complexes by Native Mass Spectrometry

Yun S, Scott E, Laganowsky A

Methods Mol Biol. 2024;2797:177-193. doi: 10.1007/978-1-0716-3822-4_13. PMID: 38570460.

DOI PubMed

202311 publications

#88

Native mass spectrometry of proteoliposomes containing integral and peripheral membrane proteins

Zhu Y, Yun SD, Zhang T, Chang JY, Stover L, Laganowsky A

Chemical Science.2023 Nov 21. doi: 10.1039/D3SC04938H.

DOI

#87

Design of a symmetry-broken tetrahedral protein cage by a method of internal steric occlusion

Gladkov N, Scott EA, Meador K, Lee EJ, Laganowsky AD, Yeates TO, Castells-Graells R

bioRxiv [Preprint]. 2023 Nov 9:2023.11.08.566319. doi: 10.1101/2023.11.08.566319.

DOI

#86

Variation of CI-2 Conformers upon Addition of Methanol to Water: An IMS-MS-Based Thermodynamic Analysis

Pan H, Raab SA, El-Baba TJ, Schrecke SR, Laganowsky A, Russell DH, Clemmer DE

J Phys Chem A. 2023 Nov 16;127(45):9399-9408. doi: 10.1021/acs.jpca.3c03651.

DOI

#85

Cardiolipin Regulates the Activity of the Mitochondrial ABC Transporter ABCB10

Zhang T, Lyu J, Zhu Y, Laganowsky A

Biochemistry. 2023 Oct 9. doi: 10.1021/acs.biochem.3c00417.

DOI

#84

Polyamine detergents tailored for native mass spectrometry studies of membrane proteins

Zhu Y, Peng BJ, Kumar S, Stover L, Chang JY, Lyu J, Zhang T, Schrecke S, Azizov D, Russell DH, Fang L, Laganowsky A

Nat Commun. 2023 Sep 14;14(1):5676. doi: 10.1038/s41467-023-41429-w.

DOI

#83

Combining native mass spectrometry and lipidomics to uncover specific membrane protein-lipid interactions from natural lipid sources

Zhu Y, Odenkirk MT, Qiao P, Zhang T, Schrecke S, Zhou M, Marty MT, Baker ES, Laganowsky A

Chem Sci. 2023 Jul 21;14(32):8570-8582. doi: 10.1039/d3sc01482g.

DOI

#82

2'-Deoxy Guanosine Nucleotides Alter the Biochemical Properties of Ras

Yun SD, Scott E, Moghadamchargari Z, Laganowsky A

Biochemistry. 2023 Aug 15;62(16):2450-2460. doi: 10.1021/acs.biochem.3c00258.

DOI

#81

Double and triple thermodynamic mutant cycles reveal the basis for specific MsbA-lipid interactions

Lyu J, Zhang T, Marty MT, Clemmer D, Russell D, Laganowsky A

bioRxiv [Preprint]. 2023 Jul 3:2023.07.03.547565. doi: 10.1101/2023.07.03.547565.

DOI

#80

Structures of Get3d reveal a distinct architecture associated with the emergence of photosynthesis

Barlow AN, Manu MS, Saladi SM, Tarr PT, Yadav Y, Thinn AMM, Zhu Y, Laganowsky AD, Clemons WM Jr, Ramasamy S

J Biol Chem. 2023 Jun;299(6):104752. doi: 10.1016/j.jbc.2023.104752.

DOI

#79

Dissecting the Thermodynamics of ATP Binding to GroEL One Nucleotide at a Time

Walker T, Sun HM, Gunnels T, Wysocki V, Laganowsky A, Rye H, Russell D

ACS Cent Sci. 2023 Feb 20;9(3):466-475. doi: 10.1021/acscentsci.2c01065.

DOI

#78

FTflow: An Open-Source Python GUI for FT-IM-MS Experiments

Cabrera ER, Laganowsky A, Clowers BH

J Am Soc Mass Spectrom. 2023 Apr 5;34(4):790-793. doi: 10.1021/jasms.2c00352.

DOI

20229 publications

#77

Structural basis for lipid and copper regulation of the ABC transporter MsbA

Lyu J, Liu C, Zhang T, Schrecke S, Elam NP, Packianathan C, Hochberg GKA, Russell D, Zhao M, Laganowsky A

Nat Commun. 2022 Nov 26;13(1):7291. doi: 10.1038/s41467-022-34905-2.

DOI

#76

High-Throughput Mass Spectrometry for Biopharma: A Universal Modality and Target Independent Analytical Method for Accurate Biomolecule Characterization

Campuzano IDG, Pelegri-O'Day EM, Srinivasan N, Lippens JL, Egea P, Umeda A, Aral J, Zhang T, Laganowsky A, Netirojjanakul C

J Am Soc Mass Spectrom. 2022 Nov 2;33(11):2191-2198. doi: 10.1021/jasms.2c00138.

DOI

#75

Step toward Probing the Nonannular Belt of Membrane Proteins

Kumar S, Zhu Y, Stover L, Laganowsky A

Anal Chem. 2022 Oct 11;94(40):13906-13912. doi: 10.1021/acs.analchem.2c02811.

DOI

#74

De novo determination of mosquitocidal Cry11Aa and Cry11Ba structures from naturally-occurring nanocrystals

Tetreau G, Sawaya MR, De Zitter E, Andreeva EA, Banneville AS, Schibrowsky NA, Coquelle N, Brewster AS, Grünbein ML, Kovacs GN, Hunter MS, Kloos M, Sierra RG, Schiro G, Qiao P, Stricker M, Bideshi D, Young ID, Zala N, Engilberge S, Gorel A, Signor L, Teulon JM, Hilpert M, Foucar L, Bielecki J, Bean R, de Wijn R, Sato T, Kirkwood H, Letrun R, Batyuk A, Snigireva I, Fenel D, Schubert R, Canfield EJ, Alba MM, Laporte F, Després L, Bacia M, Roux A, Chapelle C, Riobé F, Maury O, Ling WL, Boutet S, Mancuso A, Gutsche I, Girard E, Barends TRM, Pellequer JL, Park HW, Laganowsky AD, Rodriguez J, Burghammer M, Shoeman RL, Doak RB, Weik M, Sauter NK, Federici B, Cascio D, Schlichting I, Colletier JP

Nat Commun. 2022 Jul 28;13(1):4376. doi: 10.1038/s41467-022-31746-x.

DOI

#73

Surface Activity of Amines Provides Evidence for the Combined ESI Mechanism of Charge Reduction for Protein Complexes

Walker TE, Laganowsky A, Russell DH

Anal Chem. 2022 Aug 2;94(30):10824-10831. doi: 10.1021/acs.analchem.2c01814.

DOI

#72

Cupric Ions Selectively Modulate TRAAK-Phosphatidylserine Interactions

Zhu Y, Schrecke S, Tang S, Odenkirk MT, Walker T, Stover L, Lyu J, Zhang T, Russell D, Baker ES, Yan X, Laganowsky A

J Am Chem Soc. 2022 Apr 27;144(16):7048-7053. doi: 10.1021/jacs.2c00612.

DOI

#71

Small molecule peptidomimetic trypsin inhibitors: validation of an EKO binding mode, but with a twist

Lyu RL, Joy S, Packianathan C, Laganowsky A, Burgess K

Org Biomol Chem. 2022 Mar 9;20(10):2075-2080.

#70

Temperature Regulates Stability, Ligand Binding (Mg2+ and ATP), and Stoichiometry of GroEL-GroES Complexes

Walker TE, Shirzadeh M, Sun HM, McCabe JW, Roth A, Moghadamchargari Z, Clemmer DE, Laganowsky A, Rye H, Russell DH

J Am Chem Soc. 2022 Feb 16;144(6):2667-2678.

#64

Investigation of Protein-Lipid Interactions Using Native Mass Spectrometry

Cong X, Patrick JW, Liu Y, Liang X, Liu W, Laganowsky A

Methods Mol Biol. 2022;2349:41-64. doi: 10.1007/978-1-0716-1585-0_3.

DOI

202113 publications

#69

Charge Engineering Reveals the Roles of Ionizable Side Chains in Electrospray Ionization Mass Spectrometry

Abramsson ML, Sahin C, Hopper JTS, Branca RMM, Danielsson J, Xu M, Chandler SA, Österlund N, Ilag LL, Leppert A, Costeira-Paulo J, Lang L, Teilum K, Laganowsky A, Benesch JLP, Oliveberg M, Robinson CV, Marklund EG, Allison TM, Winther JR, Landreh M

JACS Au. 2021 Nov 29;1(12):2385-2393. doi: 10.1021/jacsau.1c00458.

DOI

#68

Variable-Temperature Native Mass Spectrometry for Studies of Protein Folding, Stabilities, Assembly, and Molecular Interactions

Laganowsky A, Clemmer DE, Russell DH

Annu Rev Biophys. 2021 Dec 21. doi: 10.1146/annurev-biophys-102221-101121.

DOI

#67

Entropy in the Molecular Recognition of Membrane Protein-Lipid Interactions

Qiao P, Schrecke S, Walker T, McCabe JW, Lyu J, Zhu Y, Zhang T, Kumar S, Clemmer D, Russell DH, Laganowsky A

J Phys Chem Lett. 2021 Dec 30;12(51):12218-12224. doi: 10.1021/acs.jpclett.1c03750.

DOI

#66

Insight into the Phospholipid-Binding Preferences of Kir3.4

Qiao P, Schrecke S, Lyu J, Zhu Y, Zhang T, Benavides A, Laganowsky A

Biochemistry. 2021 Dec 21;60(50):3813-3821. doi: 10.1021/acs.biochem.1c00615.

DOI

#65

McCabe JW, Jones BJ, Walker TE, Schrader RL, Huntley AP, Lyu J, Hoffman NM, Anderson GA, Reilly PTA, Laganowsky A, Wysocki VH, Russell DH

Implementing Digital-Waveform Technology for Extended m/z Range Operation on a Native Dual-Quadrupole FT-IM-Orbitrap Mass Spectrometer

J Am Soc Mass Spectrom. 2021 Dec 1;32(12):2812-2820. doi: 10.1021/jasms.1c00245.

DOI

#63

Mechanism-Based Inactivation of Mycobacterium tuberculosis Isocitrate Lyase 1 by (2R,3S)-2-Hydroxy-3-(nitromethyl)succinic acid

Mellott DM, Torres D, Krieger IV, Cameron SA, Moghadamchargari Z, Laganowsky A, Sacchettini JC, Meek TD, Harris LD

J Am Chem Soc. 2021 Oct 27;143(42):17666-17676. doi: 10.1021/jacs.1c07970.

DOI

#62

Self-Masked Aldehyde Inhibitors: A Novel Strategy for Inhibiting Cysteine Proteases

Li L, Chenna BC, Yang KS, Cole TR, Goodall ZT, Giardini M, Moghadamchargari Z, Hernandez EA, Gomez J, Calvet CM, Bernatchez JA, Mellott DM, Zhu J, Rademacher A, Thomas D, Blankenship LR, Drelich A, Laganowsky A, Tseng CK, Liu WR, Wand AJ, Cruz-Reyes J, Siqueira-Neto JL, Meek TD

J Med Chem. 2021 Aug 12;64(15):11267-11287. doi: 10.1021/acs.jmedchem.1c00628.

DOI

#61

Protons Are Fast and Smart; Proteins Are Slow and Dumb: On the Relationship of Electrospray Ionization Charge States and Conformations

Raab SA, El-Baba TJ, Laganowsky A, Russell DH, Valentine SJ, Clemmer DE

J Am Soc Mass Spectrom. 2021 Jul 7;32(7):1553-1561. doi: 10.1021/jasms.1c00100.

DOI

#60

Thermal Analysis of a Mixture of Ribosomal Proteins by vT-ESI-MS: Toward a Parallel Approach for Characterizing the Stabilitome

El-Baba TJ, Raab SA, Buckley RP, Brown CJ, Lutomski CA, Henderson LW, Woodall DW, Shen J, Trinidad JC, Niu H, Jarrold MF, Russell DH, Laganowsky A, Clemmer DE

Anal Chem. 2021 Jun 22;93(24):8484-8492. doi: 10.1021/acs.analchem.1c00772.

DOI

#59

Variable-Temperature Electrospray Ionization for Temperature-Dependent Folding/Refolding Reactions of Proteins and Ligand Binding

McCabe JW, Shirzadeh M, Walker TE, Lin CW, Jones BJ, Wysocki VH, Barondeau DP, Clemmer DE, Laganowsky A, Russell DH

Anal Chem. 2021 May 11;93(18):6924-6931. doi: 10.1021/acs.analchem.1c00870.

DOI

#58

Molecular assemblies of the catalytic domain of SOS with KRas and oncogenic mutants

Moghadamchargari Z, Shirzadeh M, Liu C, Schrecke S, Packianathan C, Russell DH, Zhao M, Laganowsky A

Proc Natl Acad Sci U S A. 2021 Mar 23;118(12):e2022403118. doi: 10.1073/pnas.2022403118.

DOI

#57

Covalent Inactivation of Mycobacterium tuberculosis Isocitrate Lyase by cis-2,3-Epoxy-Succinic Acid

Pham TV, Mellott DM, Moghadamchargari Z, Chen K, Krieger I, Laganowsky A, Sacchettini JC, Meek TD

ACS Chem Biol. 2021 Mar 19;16(3):463-470. doi: 10.1021/acschembio.0c00740.

DOI

#56

Selective regulation of human TRAAK channels by biologically active phospholipids

Schrecke S, Zhu Y, McCabe JW, Bartz M, Packianathan C, Zhao M, Zhou M, Russell D, Laganowsky A

Nat Chem Biol. 2021 Jan;17(1):89-95. doi: 10.1038/s41589-020-00659-5.

DOI

202013 publications

#55

Evidence for Many Unique Solution Structures for Chymotrypsin Inhibitor 2: A Thermodynamic Perspective Derived from vT-ESI-IMS-MS Measurements

Raab SA, El-Baba TJ, Woodall DW, Liu W, Liu Y, Baird Z, Hales DA, Laganowsky A, Russell DH, Clemmer DE

JACS. 2020 doi: 10.1021/jacs.0c05365.

DOI

#54

A Sec14-like phosphatidylinositol transfer protein paralog defines a novel class of heme-binding proteins

Khan D, Lee D, Gulten G, Aggarwal A, Wofford J, Krieger I, Tripathi A, Patrick JW, Eckert DM, Laganowsky A, Sacchettini J, Lindahl P, Bankaitis VA

Elife. 2020;9:e57081. doi: 10.7554/eLife.57081.

DOI

#53

Discovery of Potent Charge-Reducing Molecules for Native Ion Mobility Mass Spectrometry Studies

Lyu J, Liu Y, McCabe JW, Schrecke S, Fang L, Russell DH, Laganowsky A

Anal Chem. 2020;92(16):11242-11249. doi: 10.1021/acs.analchem.0c01826.

DOI

#52

First-Principles Collision Cross Section Measurements of Large Proteins and Protein Complexes

McCabe JW, Mallis CS, Kocurek KI, Poltash ML, Shirzadeh M, Hebert MJ, Fan L, Walker TE, Zheng X, Jiang T, Dong S, Lin CW, Laganowsky A, Russell DH

Anal Chem. 2020;92(16):11155-11163. doi: 10.1021/acs.analchem.0c01285.

DOI

#51

Insight into the Selectivity of Kir3.2 toward Phosphatidylinositides

Qiao P, Liu Y, Zhang T, Benavides A, Laganowsky A

Biochemistry. 2020;59(22):2089-2099. doi: 10.1021/acs.biochem.0c00163.

DOI

#50

Ag+ Ion Binding to Human Metallothionein-2A Is Cooperative and Domain Specific

Dong S, Shirzadeh M, Fan L, Laganowsky A, Russell DH

Anal Chem. 2020;92(13):8923-8932. doi: 10.1021/acs.analchem.0c00829.

DOI

#49

Collision-Induced Unfolding Studies of Proteins and Protein Complexes using Drift Tube Ion Mobility-Mass Spectrometer

Zheng X, Kurulugama RT, Laganowsky A, Russell DH

Anal Chem. 2020;92(10):7218-7225. doi: 10.1021/acs.analchem.0c00772.

DOI

#48

Structure and mechanism of human diacylglycerol acyltransferase 1

Wang L, Qian H, Nian Y, Han Y, Ren Z, Zhang H, Hu L, Prasa BVV, Laganowsky A, Yan N, Zhou M

Nature. 2020;581(7808):329-332. doi: 10.1038/s41586-020-2280-2.

DOI

#47

Origins of complexity in haemoglobin evolution

Pillai AS, Chandler SA, Liu Y, Signore AV, Cortez-Romero CR, Benesch JLP, Laganowsky A, Storz JF, Hochberg GKA, Thornton J

Nature. 2020;581(7809):480-485. doi: 10.1038/s41586-020-2292-y.

DOI

#46

Native IM-Orbitrap MS: Resolving What Was Hidden. Trends Analyt Chem

Poltash ML, McCabe JW, Shirzadeh M, Laganowsky A, Russell DH

2020;124:115533. doi:10.1016/j.trac.2019.05.035.

DOI

#45

Structural Analysis of the Effect of a Dual-FLAG Tag on Transthyretin. Biochemistry

Shirzadeh M, Poltash ML, Laganowsky A, Russell DH

2020;59(9):1013–1022. doi:10.1021/acs.biochem.0c00105.

DOI

#44

Melting of Hemoglobin in Native Solutions as measured by IMS-MS. Anal Chem

Woodall DW, Brown CJ, Raab SA, et al

2020;92(4):3440–3446. doi:10.1021/acs.analchem.9b05561.

DOI

#43

Enhanced Characterization of Membrane Protein Complexes by Ultraviolet Photodissociation Mass Spectrometry. Anal Chem

Sipe SN, Patrick JW, Laganowsky A, Brodbelt JS

2020;92(1):899–907. doi:10.1021/acs.analchem.9b03689.

DOI

20198 publications

#42

Variable-Temperature ESI-IMS-MS Analysis of Myohemerythrin Reveals Ligand Losses, Unfolding, and a Non-Native Disulfide Bond. Anal Chem

Woodall DW, El-Baba TJ, Fuller DR, et al

2019;91(10):6808–6814. doi:10.1021/acs.analchem.9b00981.

DOI

#41

A Conserved PLPLRT/SD Motif of STING Mediates the Recruitment and Activation of TBK1

Zhao B, Du F, Shu C, Sankaran B, Bell SL, Liu M, Lei Y, Gao X, Fu X, Fanxiu Z, Liu Y, Laganowsky, A, Zheng X, Ji J, West AP, Watson RO, Li P

Nature. 2019 May;569(7758):718-722. doi: 10.1038/s41586-019-1228-x.

DOI

#40

Intrinsic GTPase Activity of K-RAS Monitored by Native Mass Spectrometry

Moghadamchargari Z, Huddleston J, Shirzadeh M, Zheng X, Clemmer DE, M Raushel F, Russell DH, Laganowsky A

Biochemistry. 2019 Aug 6;58(31):3396-3405. doi: 10.1021/acs.biochem.9b00532.

DOI

#39

Selective binding of a toxin and phosphatidylinositides to a mammalian potassium channel

Liu Y, LoCaste CE, Liu W, Poltash ML, Russell DH, Laganowsky A

Nat Commun. 2019 Mar 22;10(1):1352. doi: 10.1038/s41467-019-09333-4.

DOI

#38

New insights into the metal-induced oxidative degradation pathways of transthyretin

Poltash ML, Shirzadeh M, McCabe JW, Moghadamchargari Z, Laganowsky A, Russell DH

Chem Commun (Camb). 2019 Apr 2;55(28):4091-4094. doi:.

#37

Generation of Charge-Reduced Ions of Membrane Protein Complexes for Native Ion Mobility Mass Spectrometry Studies

Patrick JW, Laganowsky A

J Am Soc Mass Spectrom. 2019 Mar 18. doi: 10.1007/s13361-019-02187-6.

DOI

#36

Topological Analysis of Transthyretin Disassembly Mechanism: Surface-Induced Dissociation Reveals Hidden Reaction Pathways

Shirzadeh M, Boone CD, Laganowsky A, Russell DH

Anal Chem. 2019 Feb 5;91(3):2345-2351. doi: 10.1021/acs.analchem.8b05066.

DOI

#34

Development and Evaluation of a Reverse-Entry Ion Source Orbitrap Mass Spectrometer

Poltash ML, McCabe JW, Patrick JW, Laganowsky A, Russell DH

J Am Soc Mass Spectrom. 2019 Jan;30(1):192-198. doi: 10.1007/s13361-018-1976-0.

DOI

20183 publications

#35

Fourier Transform-Ion Mobility-Orbitrap Mass Spectrometer: A Next-Generation Instrument for Native Mass Spectrometry

Poltash ML, McCabe JW, Shirzadeh M, Laganowsky A, Clowers BH, Russell DH

Anal Chem. 2018 Sep 4;90(17):10472-10478. doi: 10.1021/acs.analchem.8b02463.

DOI

#33

Allostery revealed within lipid binding events to membrane proteins

Patrick JW, Boone CD, Liu W, Conover GM, Liu Y, Cong X, Laganowsky A

Proc Natl Acad Sci U S A. 2018 Mar 5. pii: 201719813. doi: 10.1073/pnas.1719813115.

DOI

#32

Structural principles that enable oligomeric small heat-shock protein paralogs to evolve distinct functions

Hochberg GKA, Shepherd DA, Marklund EG, Santhanagoplan I, Degiacomi MT, Laganowsky A, Allison TM, Basha E, Marty MT, Galpin MR, Struwe WB, Baldwin AJ, Vierling E, Benesch JLP

Science. 2018 Feb 23;359(6378):930-935. doi: 10.1126/science.aam7229.

DOI

20175 publications

#31

Allosteric modulation of protein-protein interactions by individual lipid binding events

Cong X, Liu Y, Liu W, Liang X, Laganowsky A

Nat Commun. 2017 Dec doi: 10.1038/s41467-017-02397-0.

DOI

#30

Protonation state of glutamate 73 regulates the formation of a specific dimeric association of mVDAC1

Bergdoll LA, Lerch MT, Patrick JW, Belardo K, Altenbach C, Bisignano P, Laganowsky A, Grabe M, Hubbell WL, Abramson J

PNAS. 2017 Dec doi: 10.1073/pnas.1715464115.

DOI

#29

Melting Proteins: Evidence for Multiple Stable Structures upon Thermal Denaturation of Native Ubiquitin from Ion Mobility Spectrometry-Mass Spectrometry Measurements

El-Baba TJ, Woodall DW, Raab SA, Fuller DR, Laganowsky A, Russell DH, Clemmer DE

J Am Chem Soc. 2017 May 10;139(18):6306-6309. doi: 10.1021/jacs.7b02774.

DOI

#28

Surface induced dissociation as a tool to study membrane protein complexes

Harvey SR, Liu Y, Liu W, Wysocki VH, Laganowsky A

Chem Commun (Camb). 2017 Mar 9;53(21):3106-3109. doi: 10.1039/c6cc09606a.

DOI

#27

Characterization of Membrane Protein-Lipid Interactions by Mass Spectrometry Ion Mobility Mass Spectrometry

Liu Y, Cong X, Liu W, Laganowsky A

JASMS. 2017 Apr;28(4):579-586. doi: 10.1007/s13361-016-1555-1.

DOI

20161 publication

#26

Determining Membrane Protein-Lipid Binding Thermodynamics Using Native Mass Spectrometry

Cong X, Liu Y, Liu W, Liang X, Russell DH, Laganowsky A

J Am Chem Soc. 2016 Apr 6;138(13):4346-9. doi: 10.1021/jacs.6b01771.

DOI

20153 publications

#25

Quantifying the stabilizing effects of protein-ligand interactions in the gas phase

Allison TM, Reading E, Liko I, Baldwin AJ, Laganowsky A, Robinson CV

Nat Commun. 2015 Oct 6;6:8551. doi: 10.1038/ncomms9551. PubMed PMID: 26440106.

DOI PubMed

#24

The Effect of Detergent, Temperature, and Lipid on the Oligomeric State of MscL Constructs: Insights from Mass Spectrometry

Reading E, Walton TA, Liko I, Marty MT, Laganowsky A, Rees DC, Robinson CV

Chem Biol. 2015 May 21;22(5):593-603. doi:10.1016/j.chembiol.2015.04.016. PubMed PMID: 26000747.

DOI PubMed

#23

The role of the detergent micelle in preserving the structure of membrane proteins in the gas phase

Reading E, Liko I, Allison TM, Benesch JL, Laganowsky A, Robinson CV

Angew Chem Int Ed Engl. 2015 Apr 7;54(15):4577-81. doi: 10.1002/anie.201411622. Epub 2015 Feb 18. PubMed PMID: 25693501.

DOI PubMed

20143 publications

#22

Structure of a designed protein cage that self-assembles into a highly porous cube

Lai YT, Reading E, Hura GL, Tsai KL, Laganowsky A, Asturias FJ, Tainer JA, Robinson CV, Yeates TO

Nat Chem. 2014 Dec;6(12):1065-71. doi: 10.1038/nchem.2107. Epub 2014 Nov 10. PubMed PMID: 25411884.

DOI PubMed

#21

Membrane proteins bind lipids selectively to modulate their structure and function

Laganowsky A,Ф, § Reading E, Allison TM, Ulmschneider MB, Degiacomi MT, Baldwin AJ, Robinson CV

Nature. 2014 Jun 5;510(7503):172-5. doi: 10.1038/nature13419. PubMed PMID: 24899312. ФEqual authorship, §Corresponding author. (Cover Article).

DOI PubMed

#20

§ The structured core domain of αB-crystallin can prevent amyloid fibrillation and associated toxicity

Hochberg GK, Ecroyd H, Liu C, Cox D, Cascio D, Sawaya MR, Collier MP, Stroud J, Carver JA, Baldwin AJ, Robinson CV, Eisenberg DS, Benesch JL, Laganowsky A

Proc Natl Acad Sci U S A. 2014 Apr 22;111(16):E1562-70. doi: 10.1073/pnas.1322673111. Epub 2014 Apr 7. PubMed PMID: 24711386. §Corresponding author.

DOI PubMed

20133 publications

#19

Detergent-free mass spectrometry of membrane protein complexes

Hopper JT, Yu YT, Li D, Raymond A, Bostock M, Liko I, Mikhailov V, Laganowsky A, Benesch JL, Caffrey M, Nietlispach D, Robinson CV

Nat Methods. 2013 Dec;10(12):1206-8. doi: 10.1038/nmeth.2691. Epub 2013 Oct 13. PubMed PMID: 24122040. (Cover Article).

DOI PubMed

#18

C-terminal interactions mediate the quaternary dynamics of αB-crystallin

Hilton GR, Hochberg GK, Laganowsky A, McGinnigle SI, Baldwin AJ, Benesch JL

Philos Trans R Soc Lond B Biol Sci. 2013 Mar 25;368(1617):20110405. doi: 10.1098/rstb.2011.0405. PubMed PMID: 23530258.

DOI PubMed

#17

Mass spectrometry of intact membrane protein complexes

Laganowsky A, Reading E, Hopper JT, Robinson CV

Nat Protoc. 2013 Apr;8(4):639-51. doi: 10.1038/nprot.2013.024. Epub 2013 Mar 7. PubMed PMID: 23471109. (Cover Article).

DOI PubMed

20123 publications

#16

Dynamical coupling of intrinsically disordered proteins and their hydration water: comparison with folded soluble and membrane proteins

Gallat FX, Laganowsky A, Wood K, Gabel F, van Eijck L, Wuttke J, Moulin M, Härtlein M, Eisenberg D, Colletier JP, Zaccai G, Weik M

Biophys J. 2012 Jul 3;103(1):129-36. doi: 10.1016/j.bpj.2012.05.027. PubMed PMID: 22828339.

DOI PubMed

#15

Dimerization and heme binding are conserved in amphibian and starfish homologues of the microRNA processing protein DGCR8

Senturia R, Laganowsky A, Barr I, Scheidemantle BD, Guo F

PLoS One. 2012;7(7):e39688. doi: 10.1371/journal.pone.0039688. PubMed PMID: 22768307.

DOI PubMed

#14

Atomic view of a toxic amyloid small oligomer

Laganowsky A, Liu C, Sawaya MR, Whitelegge JP, Park J, Zhao M, Pensalfini A, Soriaga AB, Landau M, Teng PK, Cascio D, Glabe C, Eisenberg D

Science. 2012 Mar 9;335(6073):1228-31. doi: 10.1126/science.1213151. PubMed PMID: 22403391.

DOI PubMed

20113 publications

#13

Molecular basis for amyloid-beta polymorphism

Colletier JP, § Laganowsky A, § Landau M, § Zhao M, Soriaga AB, Goldschmidt L, Flot D, Cascio D, Sawaya MR, Eisenberg D

Proc Natl Acad Sci U S A. 2011 Oct 11;108(41):16938-43. doi: 10.1073/pnas.1112600108. Epub 2011 Sep 23. PubMed PMID: 21949245. §Equal authorship.

DOI PubMed

#12

An approach to crystallizing proteins by metal-mediated synthetic symmetrization

Laganowsky A, Zhao M, Soriaga AB, Sawaya MR, Cascio D, Yeates TO

Protein Sci. 2011 Nov;20(11):1876-90. doi: 10.1002/pro.727. Epub 2011 Sep 30. PubMed PMID: 21898649. Ranked “must read” in Faculty of 1000.

DOI PubMed

#11

Towards a pharmacophore for amyloid

Landau M, Sawaya MR, Faull KF, Laganowsky A, Jiang L, Sievers SA, Liu J, Barrio JR, Eisenberg D

PLoS Biol. 2011 Jun;9(6):e1001080. doi: 10.1371/journal.pbio.1001080. Epub 2011 Jun 14. PubMed PMID: 21695112.

DOI PubMed

20103 publications

#10

A novel 3-methylhistidine modification of yeast ribosomal protein Rpl3 is dependent upon the YIL110W methyltransferase

Webb KJ, Zurita-Lopez CI, Al-Hadid Q, Laganowsky A, Young BD, Lipson RS, Souda P, Faull KF, Whitelegge JP, Clarke SG

J Biol Chem. 2010 Nov 26;285(48):37598-606. doi: 10.1074/jbc.M110.170787. Epub 2010 Sep 23. PubMed PMID: 20864530.

DOI PubMed

Non-3D domain swapped crystal structure of truncated zebrafish alphaA crystallin

Laganowsky A, Eisenberg D

Protein Sci. 2010 Oct;19(10):1978-84. doi: 10.1002/pro.471. PubMed PMID: 20669149.

DOI PubMed

Crystal structures of truncated alphaA and alphaB crystallins reveal structural mechanisms of polydispersity important for eye lens function

Laganowsky A, Benesch JL, Landau M, Ding L, Sawaya MR, Cascio D, Huang Q, Robinson CV, Horwitz J, Eisenberg D

Protein Sci. 2010 May;19(5):1031-43. doi: 10.1002/pro.380. PubMed PMID: 20440841. (Cover Article).

DOI PubMed

20092 publications

Mass spectral analyses of the two major apolipoproteins of great ape high density lipoproteins

Puppione DL, Donna LD, Laganowsky AD, Bassilian S, Souda P, Ryder OA, Whitelegge JP

Comp Biochem Physiol Part D Genomics Proteomics. 2009 Dec;4(4):305-9. PubMed PMID: 21298813.

PubMed

Hydroponics on a chip: analysis of the Fe deficient Arabidopsis thylakoid membrane proteome

Laganowsky A, Gómez SM, Whitelegge JP, Nishio JN

J Proteomics. 2009 Apr 13;72(3):397-415. PubMed PMID: 19367733.

PubMed

20081 publication

Identification of two SET domain proteins required for methylation of lysine residues in yeast ribosomal protein Rpl42ab

Webb KJ, Laganowsky A, Whitelegge JP, Clarke SG

J Biol Chem. 2008 Dec 19;283(51):35561-8. doi: 10.1074/jbc.M806006200. Epub 2008 Oct 28. PubMed PMID: 18957409.

DOI PubMed

20061 publication

Sequencing covalent modifications of membrane proteins

Whitelegge JP, Laganowsky A, Nishio J, Souda P, Zhang H, Cramer WA

J Exp Bot. 2006;57(7):1515-22. Epub 2006 Mar 30. PubMed PMID: 16574746.

PubMed

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Additional Publications

Book chapters

Patrick JW, Laganowsky A. Probing Heterogeneous Lipid Interactions with Membrane Proteins Using Mass Spectrometry. Methods Mol Biol. 2019;2003:175–190.

Cong X, Patrick JP, Liu Y, Liang X, Liu W, Laganowsky A. “Investigation of Protein-lipid interactions using Native Mass Spectrometry”. In: JH Kleinschmidt, editor: Methods in Molecular Biology, Lipid-Protein interactions. 2022;2349:41-64.

Faull KF, Dooley AN, Halgand F, Shoemaker LD, Norris AJ, Ryan CM, Laganowsky A, Johnson JV, Katz JE. “An introduction to the basic principles and concepts of mass spectrometry.” In: D. Barcelo, editor: Comprehensive Analytical Chemistry, Vol 52, Protein Mass Spectrometry, Julian P. Whitelegge. Elsevier BV: Elsevier, 2009, pp. 1–46. ISBN: 9780444530554